Issue archive
Published: Kem. Ind. 61 (5-6) (2012) 289–294
Paper reference number: KUI-20/2011
Paper type: Professional paper
Download paper:  PDF

Characterization of Hydrogen Bonds by IR Spectroscopy

D. Vojta


In the identification and quantification of hydrogen bond, as one of the most abundant non-covalent interactions in phenomena like self-assembly and molecular recognition, IR spectrosopy has been employed as the most sensitive method. The performance of the high dilution method enables determination of the stability constant of hydrogen-bonded complex as one of the most important thermodynamic quantities used in their characterization. However, the alleged experimental simplicity of the mentioned method is loaded with errors originating not only from researcher intervention but also independent from it. The second source of error is particularly emphasized and elaborated in this paper, which is designed as the recipe for the successful characterization of hydrogen bonds. Besides the enumeration of all steps in the determination of hydrogen-bonded stability constants, the reader can be acquainted with the most important experimental conditions that should be fulfilled in order to minimize the naturally occurring errors in this type of investigation. In the spectral analysis, the application of both uni- and multivariate approach has been discussed. Some computer packages, considering the latter, are mentioned, described, and recommended.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License


hydrogen bond, IR spectroscopy, concentration equilibrium binding constant, Lambert-Beer law