https://doi.org/10.15255/KUI.2019.039
Published: Kem. Ind. 68 (9-10) (2019) 407–414
Paper reference number: KUI-39/2019
Paper type: Original scientific paper
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Extraction and Stability of Olive Pomace Polyphenols in Natural Deep Eutectic Solvents

A. Mitar, D. Kučić Grgić and J. Prlić Kardum

Abstract

Polyphenols, left in olive pomace, which is a by-product in the production of olive oil, are antioxidants that have a positive effect on human health. For the extraction of polyphenols, the use of natural deep eutectic solvents is increasingly encountered, characterised by non-toxicity, biodegradability, nonflammability, and low price. In this work, a natural deep eutectic solvent (NADES) was prepared from three components: malic acid, D-fructose, and glycerol (MaFruGly) with different water contents that allow successful extraction of polyphenols. The optimal extraction method was determined. Following extraction, the microbial activity was tested and stability of the isolated polyphenols monitored. Research has shown that the most successful methods for polyphenol extraction are microwave ultrasound and ultrasonic probe extraction, and NADES with 30 % water has proven to be the optimal solvent for maintaining the stability of polyphenols.

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Keywords

extraction, polyphenols, natural deep eutectic solvents, stability of polyphenols