https://doi.org/10.15255/KUI.2018.025
Published: Kem. Ind. 67 (13) (2018) P135–P142
Paper reference number: KUI-25/2018
Paper type: Conference paper
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Toxicity Determination of Xenobiotics in Water

T. Borojević, D. Ašperger and D. Dolar

Abstract

The aim of this research was to determine the toxicity of the active substances listed in the watch lists for substances that need monitoring across the EU in the field of water policy according to European Directive 2008/105/EC. Those are macrolide antibiotics, herbicides, insecticides, antioxidants, nonsteroidal antireumatics, estrogen hormones, and sunscreening agents. A quick and simple bioanalytic method was used to determine the acute ecotoxicity of xenobiotics in water by the Vibrio fischeri bacteria. These bacteria are susceptible to organic contamination, bacterial growth is inhibited, which is manifested by a reduction in their inborn luminescence. In these experiments, inhibition of bacterial luminescence was measured by adding different concentrations of xenobiotic solutions. Measurements have shown that all xenobiotics, except 2-ethylhexyl-6-methoxycinnamate, cause bacterial culture inhibition, and the most toxic xenobiotic is an insecticide, methiocarb.

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Keywords

xenobiotics, toxicity, water, Vibrio fischeri