https://doi.org/10.15255/KUI.2003.014
Published: Kem. Ind. 52 (9) (2003) 427–432
Paper reference number: KUI-14/2003
Paper type: Conference paper
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Concentration of Rosmarinic Acid with Supercritical Fluids

M. Hadolin, Ž. Knez and D. Bauman

Abstract

Rosmarinic acid is a common constituent of the Labitae. It has been found in many medicinal species of this family, such as rosemary, sage, thyme, balm, marjoram and oregano and have been shown to poses antioxidant, antiviral, antibacterial and anti-inflammatory properties. In present work, the concentration of rosmarinic acid by supercritical fluids is presented. Carbon dioxide (CO2) and dimethyl ether (DME) were used as solvents. The crude extract was prepared from rosemary with conventional extraction proces. The supercritical CO2 extraction of crude extracts was performed at presures from 100-300 bar and temperatures of 30-60 °C. Extraction with dimethyl ether (DME) was performed at presures from 100-200 bar and temperatures 30-60 °C. Effects of temperature, amount of solvent, and addition of an entrainer on the concentration of rosmarinic acid in the extracts and extraction residues were studied. Content of rosmarinic acid in the samples was determined by high performance liquid chromatography. Obtained extracts had a higher amount of rosmarinic acid, better appearance and fluid properties, no smell on esential oil, no solvent residues and were more active.

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Keywords

rosmarinic acid, natural antioxidants, high-pressure extraction, supercritical fluids