https://doi.org/10.15255/KUI.2008.035
Published: Kem. Ind. 58 (5) (2009) 207–213
Paper reference number: KUI-35/2008
Paper type: Review
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Molecular Imaging Mass Spectrometry

D. Josić and S. Kovač

Abstract

Molecular imaging mass spectrometry (IMS) is a recently developed method for direct determination of spatial distribution of biopolymers, preferably proteins on cell surface and tissues. Imaging mass spectrometry data are mainly based on Matrix-Assisted Laser Desorption/Ionization – Time of Flight (MALDI TOF). The MALDI TOF based imaging mass spectrometry was applied for determination of changes in kidney tissue of sensitive mice after poisoning with aristolochic acid I. The second application presented here were changes in the gastric tissue in mice after infection with Helicobacter pylori, as a model of gastric cancer in humans caused by this pathogen microorganism. Molecular imaging mass spectrometry can be applied in medicine, mostly for identification of candidate biomarkers for malignant and non-malignant diseases. Furthermore, imaging MS has almost unlimited capacity in agriculture, food technology and biotechnology, e. g. for monitoring, process development and quality control of manufactured tissue of animal, plant and microbial origin.

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Keywords

imaging mass spectrometry, aristolochic acid, nephropathy, gastric cancer, biomarkers