https://doi.org/10.15255/CABEQ.2015.2193

Published: CABEQ 29 (3) (2015) 351-355
Paper type: Original Scientific Paper

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Hydrothermal Degradation of Fats, Carbohydrates and Proteins in Sunflower Seeds after Treatment with Subcritical Water

M. Ravber, Ž. Knez and M. Škerget

Abstract
In this study, the hydrothermal degradation of fats, carbohydrates and proteins in sunflower seeds after treatment with subcritical water was observed. Sunflower seeds were subjected to subcritical water in a wide temperature range (130–240 °C) for periods from 5 to 120 minutes. The oil- and water-soluble phases were analysed for products of hydrothermal degradation. Oil stability was investigated by analysing the content of free fatty acids using gas chromatography. The water-soluble phase was analysed for the presence of any formed amino acids, and the amount of carbohydrates remaining after treatment was determined. Total amino acids and carbohydrates were determined using the ninhydrin and phenol/sulphuric acid spectrophotometric methods, respectively. The results show that oils are the most stable macronutrient present in sunflower seeds. Only small amounts of free fatty acids had formed during processing but the amount drastically started to increase at 240 °C. Proteins seem to be less stable than oils, whereas carbohydrates have proven to be very susceptible to hydrothermal degradation.


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Keywords
subcritical water, hydrothermal degradation, free fatty acids, carbohydrates, proteins