Poly(hydroxyalkanoate) Production by Cupriavidus necator from Fatty Waste Can Be Enhanced by phaZ1 Inactivation

S. Povolo, M. Basaglia, F. Fontana, A. Morelli and S. Casella

Abstract
PHA production from waste oils or fats requires microorganisms that should be both excellent PHA producers and equipped with enzymatic activities allowing hydrolysation of triglycerides. Unfortunately, microbes with the combination of substrate-utilization and PHA production are not currently available, and the strategies to be adopted are the use of costly commercial enzymes, or genetic modification of microorganisms exhibiting high PHA product yields. In the present work, after a general investigation on the ability of Cupriavidus necator to grow on a number of fatty substrates, the possibility to enhance PHA production by limiting intracellular depolymerisation, was investigated. By knocking out the related phaZ1 gene, the construction of C. necator recombinant strains impaired in depolymerase (PhaZ1) activity was achieved. The polymer yield of the recombinant strain was finally compared to that of the parental C. necator DSM 545.

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Keywords
poly(hydroxyalkanoates), glycerol, lipase activity, depolymerase, Cupriavidus necator