Repeated Fed-batch Sorbose Fermentation by Gluconobacter oxydans

R. Giridhar and A. K. Srivastava

Abstract
Gluconobacter oxydans was cultivated in repeated fed-batch culture for the bioconversion of D-sorbitol to L-sorbose. Batch culture with an initial concentration of 200 kg m–3 sorbitol was converted to repeated fed-batch by harvesting one-third of the reactor volume and recharging with fresh nutrient medium having the same sorbitol concentration. Four cycles of harvesting and recharging were done. At the end of fourth cycle (26 h) 2.26 kg of sorbose was produced with an increased average productivity of 19.31 kg m–3 h–1.

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Keywords
Gluconobacter oxydans, fed-batch, sorbitol, sorbose