Adaptive Control of Saccharomyces cerevisiae Yeasts Fed-Batch Cultivations

J. Vovsík, P. Hrnčiřík, J. Náhlík and J. Mareš

Abstract
In this paper, the application of an adaptive algorithm for control of fed-batch bioprocess capable of coping with time-variant process properties in the presence of uncertainties is introduced. The proposed adaptive controller uses Maršík’s heuristic algorithm for adaptation based on control error oscillation rate criterion without the need of a mathematical model of the controlled process or any special test signals. The intended application of the resulting controller was off-gas CO2 concentration control in fed-batch yeast cultivations where the set point has the form of a time-varying concentration profile. The controller has been tested in a series of experimental fed-batch cultivations with D7 Saccharomyces cerevisiae strain, a UV mutant suitable for ergosterol production, in 7‑litre laboratory bioreactor. Obtained results demonstrate good properties of this adaptive controller that can be used without the need for a tedious parameter identification of the complex bioprocess.

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Keywords
Adaptive control, identification-free, fed-batch cultivation, baker’s yeast, Saccharomyces cerevisiae