Optimization of pH and Temperature in the Process of Bioconversion of Glucose to 2,5-diketo-D-gluconic Acid

B. Zelić, N. Pavlović, V. Delić and Đ. Vasić-Rački

Abstract
This work presents the optimization of pH and temperature for biomass growth. A two-level factorial experimental design with central point was used. The evolutionary operation method (EVOP) was used to determine optimal operating conditions. A numerical algorithm explained below determined maximum specific growth rate, as a trial function. Optimal operating conditions were found to be pH 5.5 and the temperature 32 °C.

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Keywords
Bioconversion process, Erwinia citreus, 2,5-diketo-D-gluconic acid, EVOP method