Recovery of a Recombinant Thermostable Endoglucanase from E. Coli Using Supercritical Carbon Dioxide Cell Disruption

T. Juhász, E. Székely, B. Simándi, Z. Szengyel and K. Réczey

Abstract
For the production of thermostable endoglucanase from Clostridium thermocellulum the celC gene was cloned into Escherichia coli BL 21 (DE3) host. The recombinant E. coli was grown in shake flask cultures. The intracellular recombinant protein was extracted from the cells after applying supercritical CO2 cell disruption. The supercritical CO2 cell disintegration was optimized and then compared to the traditional ultrasonic cell disruption technique. With the supercritical cell disruption the cellulase recovery was approximately 17 % lower than that of obtained with sonication.

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Keywords
Cell disintegration, Clostridium thermocellum, recombinant E. coli, supercritical carbon dioxide, sonication, thermostable endoglucanase