Optimization of Process Variables for the Extractive Fermentation of 2,3-Butanediol by Klebsiella oxytoca in Aqueous Two-phase System Using Response Surface Methodology

S. G. and T. Swaminathan

Abstract
Aqueous two-phase (ATP) extractive fermentation is a novel technique which can overcome some of the limitations of the conventional fermentation and yield high productivities. Temperature, pH and agitation, are some of the key process parameters which play an important role in fermentation. Optimizing of the parameters for the ATP fermentation for 2,3-butanediol production was studied. Statistically designed experiments using response surface methodology was used to get more information about interactions between the parameters. A 23 full factorial central composite design followed by multi-stage Monte-Carlo optimization technique was employed for experimental design and analysis of the results. The optimum process conditions for the enhanced production of 2,3-butanediol in ATP system were as follows: temperature T = 31.5 °C, pH 6.7 and agitation, n = 172 rpm. Maximum average productivity of 2,3-butanediol was achieved (0.74 kg m–3 h–1) at the optimum process conditions.

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Keywords
Klebsiella oxytoca, 2,3-butanediol, aqueous two-phase system, statistical optimization, extractive fermentation