Xylanase Production by Penicillium citrinum in Laboratory-scale Stirred Tank Reactor

G. Ghoshal, U. C. Banerjee and U. S. Shivhare

Abstract
Xylanase constitutes an important class of hydrolases, and is used in numerous industrial applications. The aim of the present work was to study the production of xylanase from Penicillium citrinum MTCC 9620 in a 5 L stirred tank bioreactor. Effect of various process parameters; pH, temperature, aeration, agitation rates, substrate concentration, and, dissolved oxygen (DO) concentration on xylanase production were studied. Combination of all the optimized parameters resulted in 2.5 times higher enzyme activity as compared to the shake flask fermentation after 96 h. Effect of varying agitation and aeration on the volumetric mass transfer coefficient (KLa) was determined. It revealed that KLa is influenced by both aeration and agitation. Growth kinetics of P. citrinum MTCC 9620 in bioreactor was studied using Monod, Moser, Contois and Edward equation. Based on R2, SE and pattern of residuals, the microbial growth kinetics of P. citrinum MTCC 9620 was effectively represented by Moser equation.

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Keywords
xylanase, Penicillium citrinum, sugarcane bagasse, volumetric mass transfer coefficient, kinetics models