Examination of Cellulase Enzyme Production by Trichoderma Reesei Rut C30 Using Supercritical Carbon Dioxide Cell Disruption

A. Egyházi, D. Dienes, K. Réczey and B. Simándi

Abstract
Cellulase enzyme production by Trichoderma reesei RUT C30 was examined on two different carbon sources, delignified pine pulp (Solka Floc, SF) and glucose. In order to determine the total amount of enzymes produced, besides measuring the extracellular cellulase and β-glucosidase activities, intracellular enzyme activities were also determined. Cell disruption was performed using supercritical carbon dioxide. Cellulase activity could be detected only on SF, which is an inducer of cellulase production, whereas equal amounts of β-glucosidase activity could be observed on both carbon sources. When examining the „total” (extra and intracellular) cellulase and β-glucosidase activities achieved on SF carbon source, a ratio of 1:1.14 was found. This means that in the cell an optimal ratio of enzymes is produced but β-glucosidase is partly cellbound. The results also emphasise the difference in the production of -glucosidase and the other components of the cellulase enzyme complex, that is, while the endo- and exoglucanases are inductive enzymes, β-glucosidase enzyme is produced constitutively.

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Keywords
cellulase, Trichoderma, supercritical, Carbon dioxide, disruption