Published: CABEQ 19 (2) (2005) 199–205
Paper type: Original Scientific Paper

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A Novel Process of Purifying Soybean Hull Peroxidase

J. Liu, Y. Zhang, L. Qiu, L. Ye, Y. Xia and Z. Su

The separation and purification method of peroxidase was studied. A novel and simple purification process was used. RZ value of soybean hull peroxidase (SBP) reached 1. 32 and the recovery of enzyme activity was 65 % after purification. The new process is: the enzyme solution was purified by the ammonium sulphate–acetone cooperation precipitation, the acetone precipitation and zinc sulfate precipitation. The ammonium sulphate–acetone cooperation precipitation, namely, the two-phase system of organic solvent- inorganic salt, was adopted in our process. This is a simple and effective purification method for biomacromolecule.

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Soybean hull peroxidase (SBP), purification, cooperation precipitation, acetone fractional precipitation