Modeling of the Biotransformation Processes

A. Vrsalović Presečki, Z. Findrik Blažević and B. Zelić

Abstract
Modeling and simulation of biotransformation processes have a large potential in searching for optimal process conditions, development and process design, control, scale-up, identifying of the process cost structure, and comparing process alternatives. Modeling and simulation leads to better understanding and quantification of the investigated process and could lead to significant material and costs savings especially in the early phases of the process development. In this review modeling and simulation techniques are demonstrated on two basically different types of bioprocesses, enzymatic and microbial biotransformations. Acetophenone reduction catalyzed by ADH from Thermoanaerobacter sp., amino acid oxidation catalyzed by D-amino acid oxidase from Arthrobacter protophormiae, and L-DOPA oxidation catalyzed by L-amino acid oxidases from Crotalus adamanteus and Rhodococcus opacus are examples for modeling of enzymatic biotransformation processes. On the other hand, microbial biotransformation processes are shown for: production of alcohol dehydrogenase (ADH) in baker's yeast growing cells, production of L-malic acid by permeabilized non-growing yeast cells, production of 2,5-diketo-D-gluconic acid using Pantoea citrea, and for Escherichia coli based pyruvate production.

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Keywords
modeling, enzymatic biotransformation, microbial biotransformation