Production of Gibberellic Acid by Multiple Fed-batch Cultivation of Gibberella fujikuroi

R. Shukla, S. Chand and A. K. Srivastava

Abstract
The growth of Gibberella fujikuroi and kinetics of gibberellic acid production was studied in a multiple fed-batch cultivation process at pH 5.0 and 30 °C. Application of four nutrient addition cycles of fed-batch mode of operation was used to extend the batch fermentation and increase the final mass concentration of biomass and gibberellic acid. The lag period was totally eliminated in the subsequent feeding of 25 % nutrient feeding and simultaneous withdrawal of the fermentation broth. At the end of the fourth cycle, a biomass concentration of γ = 12.2 kg m–3 and gibberellic acid mass concentration of γ = 1.31 kg m–3 were obtained. This cultivation methodology enhanced significantly the growth and production phase of the culture under non-limiting and non-inhibitory conditions, and may be performed on a continuous basis until the batch is contaminated.

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Keywords
Multiple fed-batch fermentation, Gibberellic acid, Gibberella fujikuroi