Published: CABEQ 22 (1) (2008) 119–124
Paper type: Original Scientific Paper

Download PDF

Enhanced Production of Alkaline Protease by Teredinobacter turnirae in Fed-Batch Fermentation using a Concentrated Medium

U. Beshay

To increase cell concentration and the production of alkaline protease, fed-batch cultures with standard medium (1x) or concentrated medium (2x or 5x) as feed were performed. In a fed-batch culture with standard medium as feed, the biomass concentration reached 9.6 g L–1 and the protease activity obtained was 8500 U mL–1 compared to 3000 U mL–1 obtained by growing Teredinobacter turnirae cells with standard PB-medium in batch culture. For further increase of process efficiency, a high density fed-batch process was developed, where concentrated medium is fed to the cells. Fed-batch process using concentrated medium was evaluated for its ability to improve cell growth and protease titer of T. turnirae. It was found to result in up to 3.9-fold increase in final protease activity compared to batch culture control.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License

Teredinobacter turnirae, Alkaline protease, fed-batch culture, concentrated medium