Statistical Screening of Supplementary Nitrogen Source for Enhanced Production of L-Asparaginase by Aspergillus terreus 1782

G. Baskar, C. Sriharini, R. Sripriya and S. Renganathan

Abstract
In the present work, Latin Square Design (LSD) was applied to find the best supplementary nitrogen source for extracellular L-asparaginase production by Aspergillus terreus MTCC 1782 using corn flour as substrate in submerged fermentation. The independent effect of supplementary nitrogen source such as ammonium chloride, urea and sodium nitrate was studied on growth and production of L-asparaginase by A. terreus. Statistical significance of the nitrogen sources was studied by performing analysis of variance (ANOVA) and graphical ANOVA for LSD using Data plot software. It was found that there is no significant difference on growth due to the change in supplementary nitrogen source studied. Urea was identified as best supplementary nitrogen source with mean L-asparaginase production of 33.25 IU mL–1 and mean biomass production of 12.99 mg mL–1.

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Keywords
L-Asparaginase activity, submerged fermentation, latin square design, supplementary nitrogen source