Application of Experimental Design and Canonical Analysis of Response Surfaces to the Optimization of Poly(3-hydroxyalkanoates) Production by Pseudomonas aeruginosa 42A2

E. Rodríguez-Carmona, A. Manresa and J. Bastida

Abstract
The aim of this work was to optimize the culture medium for biomass and PHA production by Pseudomonas aeruginosa 42A2, using a five-level-three-factor central composite rotatable design (CCRD) combined with response surface methodology (RSM). A total of 23 sets of experiments was developed to obtain the second-order polynomial equations that were used to predict biomass and PHA production in terms of three independent variables: carbon source, nitrate and phosphate concentrations. Canonical analysis of the response surface models showed that the optimum medium composition differed for the production of biomass and PHA. When the models were validated experimentally by culturing Pseudomonas aeruginosa 42A2 in the optimal media, similar values to the predicted ones were obtained for the biomass (18.73 g L–1) and PHA (4.52 g L–1) concentrations. Moreover, central composite rotatable design and canonical analysis of the response surfaces proved to be useful tools for determining the optimum composition of the culture medium.

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Keywords
Bioprocessing, fermentation, optimisation, response surface methodology, canonical analysis, polyhydroxyalkanoates