Isolation and Performance Study of a Novel Lignin-degrading Strain

W. Zhang and Z. Liu

Abstract
The degradation of lignin cannot only reduce the impact of lignin pollutants on the environment, but it can also help to realize the efficient utilization of biomass resources. Obtaining the efficient lignin-degrading strain is necessary for the application of lignin biological degradation treatment. In this study, a novel lignin-degrading strain M01-L2 was isolated from a forest park in eastern China. The sequencing results showed that the M01-L2 strain was Burkholderia sp. H801. The kraft lignin degradation experiments showed that the lignin degradation efficiency of M01-L2 reached 49.8 % on the sixth day with the initial lignin concentration of 1 g L–1. This degradation process was carried out in the medium with lignin as the only carbon source. The lignin degradation process by M01-L2 strain mainly depended on the action of Lac and MnP enzymes. The MnP activity was maintained at a high level and increased significantly in the first four days, and the maximum value was 1544.4 U L–1. Lac enzyme activity increased significantly from the third day to the sixth day, reaching 757.9 U L–1 on the sixth day. This study can provide a new method for bacterial degradation of lignin.

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Keywords
lignin degradation, Kraft lignin, Lac, MnP