Published: CABEQ 35 (1) (2021) 65-80
Paper type: Original Scientific Paper

Download PDF

Model-based Optimization of Biopolymer Production from Glycerol

K. Sharmaa, A. K. Srivastava and T. R. Sreekrishnan

The present study focuses on sustainable production of biodegradable polymers by Cupriavidus necator DSMZ 545 using glycerol as substrate. The batch growth and biopolymer production kinetics were established in a 7-L bioreactor, which resulted in a total biomass of 8.88 g L–1 and poly(3-hydroxybutyrate) (PHB) accumulation of 6.76 g L–1. The batch kinetic and independently acquired substrate inhibition data were then used to develop a mathematical model for PHB production process. This was eventually used to design different nutrient feeding strategies under constant feed rate, decreasing feed rate, and pseudo steady state of substrate (glycerol) to optimize the PHB production during fed-batch cultivation. Among all the fed-batch cultivation strategies, the highest PHB accumulation and productivity of 13.12 g L–1 and 0.27 g L–1 h–1, respectively, was achieved in fed-batch bioreactor cultivation where a pseudo steady state with respect to glycerol was maintained.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License

poly(3-hydroxybutyrate), Cupriavidus necator, glycerol, mathematical model, fed-batch cultivation strategies