Effect of Developed Low Cost Minimal Medium on Lipid and Exopolysaccharide Production by Lipomyces starkeyi Under Repeated Fed-batch and Continuous Cultivation

V. Thirumal, A. Chistoserdov, R. Bajpai, J. Bader, M. K. Popovic and R. Subramaniam

Abstract
The main objective of this study was to investigate the effects of a low cost minimal medium, developed by the UL Bioprocessing Lab, on the cultivation of Lipomyces starkeyi NRRL Y-11557 using repeated fed-batch and continuous fermentation strategies. The highest cell and lipid concentrations obtained were 22.7 g L–1 and 11.67 g L–1 under repeated fed-batch cultivation, respectively. Continuous cultivation with the dilution rate of 0.06 h–1 presented the highest cell (0.401 g g–1) and lipid yields (0.177g g–1). Exopolysaccharide production was observed when L. starkeyi was cultivated in the minimal media supplemented with 90 g L–1 glucose under repeated fed-batch fermentation. The produced exopolysaccharide is likely composed of 4–5 repeating sugar units, incorporating mannose and galactose and their respective uronic acids. (This work is licensed under a Creative Commons Attribution 4.0 International License.)

This work is licensed under a Creative Commons Attribution 4.0 International License

Keywords
minimal medium, Lipomyces starkeyi, lipid, exopolysaccharide, fed-batch mode, continuous mode