Published: CABEQ 32 (2) (2018) 257ā266
Paper type: Original Scientific Paper
V. Anitha, K. Abinaya, S. Prakash, A. Seshagiri Rao and V. Balakrishnan
Abstract
In the present study, a potential bacterial strain with maximum urease activity was
isolated from urea-rich paddy field soil for biocement production. The bacterial isolate
was screened using Christensen selective agar media and named as KLUVAA. This isolate was found to be tolerant up to 10 % urea. 16S rRNA sequencing analysis identified
the isolate KLUVAA as Bacillus cereus. Biocement production was carried out using tap
water with 431.7 mg Lā1 of hardness as a natural source of calcium. Functional groups
present in biocement were analysed using FT-IR spectrum. The morphology and elemental composition of the biocement was studied using SEM with EDS mapping and XRD analyses. Thermogravimetric analysis was used to study the thermal stability of the microbial biocement. Further, process parameters were optimized for enhancing the yield of biocement.
(This work is licensed under a Creative Commons Attribution 4.0 International License.)
This work is licensed under a Creative Commons Attribution 4.0 International License
Keywords
Bacillus cereus, biocement, calcium carbonate, hard water, urease