Covalent Immobilization and Characterization of Penicillin G Acylase on Magnetic NiFe2O4 Nanorods Prepared via a Novel Rapid Combustion Process

R. Liu, Y. Li, J. Han and H. X. Fu

Abstract
The surface of magnetic NiFe2O4 nanorods prepared via a novel rapid combustion process was modified by sodium silicate and glutaraldehyde, and the penicillin G acylase (PGA) was successfully immobilized on the surface-modified magnetic NiFe2O4 nanorods. The properties of immobilized PGA were investigated, and the results showed that the immobilized PGA with high effective activity, good stability of enzyme catalyst, and good reusability was less affected by pH and temperature than free PGA. The immobilized PGA on magnetic NiFe2O4 nanorods could be separated easily from the reaction solutions by the external magnetic field for cyclic utilization, and they could retain over 70 % of initial enzyme activity after 10 consecutive operations. All the results suggested that the surface-modified magnetic NiFe2O4 nanorods would be promising for applications in the biomaterial fields.

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Keywords
NiFe2O4 nanorods, rapid combustion process, penicillin G acylase, immobilization